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Home / Proteins & Peptide

PKC iota/lambda, GST-tag

SKU : BHP18200136

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Mfr.No.	40015
Description	Human PKC iota (PCKiota), also known as PKClambda (PKClambda), GenBank Accession No. NM_002740, a.a. 2-587 with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW= 93 kDa. host species: Sf9 cells Host species: Sf9 cells Specific activity: 35 U/ug Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 7 peptide substrate per minute at pH 7.4 and 30 °C.
Background	PKCiota (PKC iota) is a member of the protein kinase C family of serine-threonine kinases. The amino acid sequence of PKCiota showed greatest homology to PKCzeta (PCKzeta), with 72% identity overall rising to 84% in the catalytic domain. Protein kinase Ciota (PKCiota) has been implicated in Ras signaling and is a critical downstream effector of oncogenic Ras in the colonic epithelium. Transgenic mice expressing constitutively active PKCiota in the colon are highly susceptible to carcinogen-induced colon carcinogenesis.
Immunogen Region	2-587
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3mM DTT and 50% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA and 1 mM DTT) containing 20 uM ATP and 0.2 mg/ml MBP for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega). Avoid freeze/thaw cycles.
GenBank	NM_002740
Mw(kda)	93 kDa

Synonyms	Protein Kinase C Iota, PCKi, PKClambda, Protein Kinase C Lambda, PCKl, PKCiota, PKClambda
Uniprot	P41743

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Zhang, J. et al., J. Biol. Chem. 279, 22118- 22123 (2004). 2. Gustrafson, W.C. et al., J. Biol. Chem. 279, 9400-9408 (2004).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200136	40015	10 ug	$390.50		Add to Cart

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