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Home / Proteins & Peptide

PI3 kinase (p110beta/p85alpha), FLAG-tag

SKU : BHP18200280

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BPS Bioscience

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Mfr.No.	40622
Description	Complex of N-terminal FLAG-tagged recombinant full-length human p110beta (GenBank Accession No. NM_006219), and untagged, recombinant full length, human p85alpha. (GenBank Accession No. NM_181523). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110beta MW=124 kDa, p85alpha MW=84 kDa. Host cell line: Sf9 cells Specific activity: 1023 pmol/min/ug Unit definition: A unit of p110beta/p85alpha kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3,4,5-Trisphosphate/min at 30°C.
Immunogen Region	full length
Tag	N-terminal FLAG-tag(p110beta), no tag on p85alpha
Formulation	25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, 50% glycerol, and 0.05 mg/ml FLAG peptide.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 25 ul kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl₂, 100 uM ATP, 200 uM PI:PS substrate (BPS catalog #40560) and 110beta/p85alpha enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo Kinase Assay Platform (Promega Corporation, Madison, WI). Avoid freeze/thaw cycles.
GenBank	NM_006219 (p110beta), NM_181523 (p85alpha)
Mw(kda)	208 kDa (complex)

Synonyms	PIK3CB, p110beta, Phosphoinositide-3-Kinase, Catalytic, Beta, PIK3R1, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), GRB1, p85alpha
Uniprot	p110ß: P42338; p85alpha: P27986

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Marques M. et al., (2008). Mol. Cell. Biol. 28 (8), 2803-2814. 2. Straub A. et al., (2008). Thromb. Haemost. 99 (3), 609-615.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200280	40622	20 ug	$467.50		Add to Cart

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