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Home / Proteins & Peptide

PHGDH, His-tag

SKU : BHP18200566

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Mfr.No.	71079
Description	Human phosphoglycerate dehydrogenase (PGDH), also known as PDG, a.a. 1 - 533 (end) (GenBank Accession No. NM_006623) with C-terminal His-tag, MW = 57.5 kDa, expressed in an E. coli expression system. Specific activity: >=80 pmol/min/ug
Immunogen Region	1 - 533 (end)
Tag	C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 170 mM imidazole, 0.04% Tween-20, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl, 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid (Sigma P8877), 1 mM NAD, and various amount of PHGDH in a final volume of 50 uL. Reactions were initiated with the addition of PHGDH, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature. Avoid freeze/thaw cycles.
GenBank	NM_006623
Mw(kda)	57.5 kDa

Synonyms	phosphoglycerate dehydrogenase, PGDH, PDG
Uniprot	O43175

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Mullarky, E., et al., Pigment Cell Melanoma Res. 2011 Dec;24(6):1112-5. 2. Liu, J., et al., J Neurooncol. 2013 Feb;111(3):245-55.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200566	71079	100 ug	$440		Add to Cart

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