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Home / Proteins & Peptide

PFKFB3, His-tag

SKU : BHP18200572

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BPS Bioscience

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Mfr.No.	71089
Description	Human 6-phosphofructo-2-kinase/ fructose-2,6-biphosphatase 3, also known as PFKFB3 and IPFK2, (GenBank Accession No. NM_004566), a.a. 2-520(end)) with N-terminal His-tag, MW = 60.4 kDa, expressed in an E. coli cell expression system. Specific activity: >=28.5 pmole/min/ug.
Immunogen Region	2-520(end)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 360 mM imidazole and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	The reaction was done in 50 mM Tris-HCl, pH 7.5, 12.5 mM MgCl2, 1 mM beta-D-fructose-6-phosphate, 100 uM ATP, 2 mM DTT, 5 mM KH2PO4, and various amount of PFKFB3 in a final volume of 50 uL. The reaction was incubated at 30°C for 30 min, and the amount of ATP consumed was calculated using Kinase-Glo Plus reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_004566
Mw(kda)	60.4 kDa

Synonyms	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3, PFKFB3, PFK2, IPFK2
Uniprot	Q16875

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Cavalier, M.C., et al., Proteins 2012;80(4):1143-1153. 2. Reddy, M.M., et al., Leukemia 2012;26(3):481-489.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200572	71089	50 ug	$407		Add to Cart

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