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Home / Proteins & Peptide

pf-HDAC1, Malaria, His-tag, FLAG-tag

SKU : BHP18200327

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BPS Bioscience

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Mfr.No.	50063
Description	Malarial (Plasmodium falciparum) HDAC, Genbank Accession No. AAD22407.1, full length (1-450) with C-terminal His-tag and FLAG-tag, MW=53.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: >=1.85 pmol/min/ug
Immunogen Region	1-450
Tag	C-terminal His-tag and FLAG-tag
Formulation	45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 90 ug/ml FLAG peptide, 10% glycerol.
Species	Malaria (Plasmodium falciparum)
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 uM BPS HDAC substrate (Cat. #50037), and 2 ng/ul pfHDAC. HDAC reaction was performed for 30 min at 37°C followed by treatment with 50 ul of HDAC developer (Cat. #50030) for 15 min at RT. Fluorescence intensity is measured at ex360/em460. Avoid freeze/thaw cycles.
GenBank	AAD22407.1
Mw(kda)	53.4 kDa

Synonyms	histone deacetylase 1 plasmodium, falciparum, pf-HDAC-1 Malaria
Uniprot	Q7K6A1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Chen et al., J. Med. Chem. 2008 Jun 26; 51(12):3437-48. 2. Joshi et al., Mol. Biochem. Parasitol. 1999 Mar 15; 99(1):11-9.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200327	50063	20 ug	$484		Add to Cart

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