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Home / Proteins & Peptide

PEST (PTPN12), GST-tag

SKU : BHP18200035

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Mfr.No.	30023
Description	Human recombinant PTP-PEST, also known as PTPN12 (GenBank Accession #NM_002835), a.a. 2-300 (catalytic domain) with N-terminal GST tag, MW=61.4 kDa, expressed in an E. coli expression system. Specific activity: 76 pmole/min/ug
Background	Protein tyrosine phosphatase-PEST (PTPN12), a ubiquitously expressed cytoplasmic tyrosine phosphatase, is thought to play an important role in cell adhesion and motility, cell migration, and signal transduction for antigen receptors in B and T lymphocytes. Signal transduction via tyrosine phosphorylation, normally fine-tuned by the concerted action of both protein tyrosine kinases and protein tyrosine phosphatases (PTPs), is a key mechanism in tumorigenesis. Studies suggest potential role for PTP-PEST in regulation of p130(cas) in mitogen- and cell adhesion-induced signaling events.
Immunogen Region	2-300
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Notes	20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 2 ng/ul PEST. Assay was done at RT for 15 min, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on epsilon_pNPP = 18,000 M^-1cm^-1. Avoid freeze/thaw cycles.
GenBank	NM_002835
Mw(kda)	61.4 kDa

Synonyms	PTP-PEST, PTPG1, tcag7.1075, PTPN12
Uniprot	Q05209

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	>12 months at -80°C

Reference	1. J.F. Cote, et al., J Biol Chem. 2002, 277: 2973-86. 2. K. Horsch et al., Mol Endocrinol. 2001, 12: 2182-96.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200035	30023	50 ug was 20 ug	$357.50		Add to Cart

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