JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

PDK1, GST-tag

SKU : BHP18200158

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	40080
Description	Human 3-phosphoinositide-dependent protein kinase (PDK1), GenBank Accession No. NM_002613, full length with N-terminal GST-tag, MW=89 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=100 pmol/min/ug
Background	PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2. PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4, 5)P3 and/or PtdIns(3,4)P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in Kinase buffer containing 0.2 mM DTT using PDKtide substrate (0.1 mg/ml) and 20 uM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was measured using Kinase-Glo reagent (Promega) Avoid freeze/thaw cycles.
GenBank	NM_002613
Mw(kda)	89 kDa

Synonyms	PRO0461; PDPK1; MGC20087; MGC35290; PkB-like; PkB-like 1 ,
Uniprot	O15530

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Yang, KJ., et al., J Biol Chem. 2008 Jan 18,283(3):1480-91. 2. Kikani, CK., et al., J Cell Biochem. 2005 Dec 15,96(6):1157-62.Application Reference: PDK1 controls upstream PI3K expression and PIP3 generation (2014)

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18200158	40080	10 ug	$390.50		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.