PDHK1, also known as Pyruvate Dehydrogenase Kinase or PDK-1 (GenBank Accession No. NM_002610), a.a. 2-437(end), with an N-terminal GST-tag, MW=71.8 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
host species: Sf9 cells
Host species: Sf9 cells
Immunogen Region
2-437(end)
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Avoid freeze/thaw cycles.
GenBank
NM_002610
Mw(kda)
71.8 kDa
Synonyms
PDHK1, Pyruvate Dehydrogenase Kinase, PDK-1
Uniprot
Q92902
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Wigfield, S.M., et al. (2008). Brit. J. Cancer. 98(12): 1975-84. 2. Sugden, M.C., et al. (2000). Biochem. J. 352(3): 731-738. 3. Kim, J.W., et al., (2006). Cell Metab. 3(3):177-85.