Human PDGFRbeta (GenBank Accession No. NM_002609), cytoplasmic domain, a.a. 558-1106(end) with N-terminal His-tag, MW= 62 kDa, expressed in Sf9 cells via a Baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 15 pmol/min/ug.
Immunogen Region
558-1106(end)
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
The enzyme reaction was carried out for 1 hour at 30°C in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 uM of ATP. Substrate peptide: Tyr2 peptide from Invitrogen.
1. Claesson-Welsh L. et al. (1988). Mol. Cell. Biol. 8:3476. 2. Heldin C.-H. and Westermark B. (1999). Physiol. Rev. 79:128. 3.Soriano, P. Genes Dev. 1994 Aug 15,8(16):1888-96. 4. Xu, L. et al. Cancer Res. 2005 Jul 1,65(13):5711-9.