Human recombinant PDGF-BB (GenBank Accession No. NM_002608) is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total a.a.) expressed in an E. coli expression system.
Reconstitution
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Immunogen Region
82-190
Tag
none
Formulation
Lyophilized from a 0.2 um filtered 0.02M acetic acid solution, pH 6.5.
Species
Human
Application
Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Endotoxin Level
< 1EU per ug of PDGF-BB as determined by the LAL method.
Notes
Avoid freeze/thaw cycles.
Biological Activity Comment
The ED50 as determined by the dose-dependent stimulation of BALB/c 3T3 cells proliferation is< 1.0 ng/ml.