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Home / Proteins & Peptide

PDE9A2, GST-tag

SKU : BHP18200529

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BPS Bioscience

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Mfr.No.	60090
Description	Human PDE9A2 (GenBank Accession No. NM_001001567), full length with N-terminal GST-tag, MW=87.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=200 pmol/min/ug. Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 16 mMglutathione, 3 mM DTT and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE9A kit, Cat. # 60390. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 200 uM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE9A2 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the5' -GMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_001001567
Mw(kda)	87.7 kDa

Synonyms	PDE9A, phosphodiesterase 9A
Uniprot	O76083

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Rentero C. and Puigdomenech P., BMC Mol. Biol. 7, 39 (2006). 2. Huai, Q., et al., Journal Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9624-9629 (2004).Application Reference(s): 1.Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015) 2.Active Site Coupling in PDE:PKA Complexes Promotes Resetting of Mammalian cAMP Signaling (2014) 3.Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014) 4.A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013) 5.Analysis of substrate specificity and kinetics of cyclic nucleotide phosphodiesterases with N'-methylanthraniloyl-substituted purine and pyrimidine 3',5'-cyclic nucleotides by fluorescence spectrometry (2013) 6.The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200529	60090	10 ug	$500.50		Add to Cart

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