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Home / Proteins & Peptide

PDE8A1, GST-tag

SKU : BHP18200528

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BPS Bioscience

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Mfr.No.	60080
Description	Human PDE8A1 (GenBank Accession No. NM_002605), full length with N-terminal GST-tag, MW= 119 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=878 pmol/min/ug. Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris pH 8.0, 110 mMNaCl, 2.2mM KCl, 0.04% Tween-20, 20%glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE8A kit, Cat. # 60380. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 0.1 mg/ml BSA, 200 uM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE8A1 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_002605
Mw(kda)	119 kDa

Synonyms	PDE8A, phosphodiesterase 8A
Uniprot	O60658

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Wu P. and Wang P. Proc. Natl. Acad. Sci. U.S.A. 101 (51), 17634-17639 (2004). 2. Gamanuma M. et al., Cell. Signal. 15 (6), 565-574 (2003).Application Reference(s): 1.Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015) 2.Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014) 3.A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013) 4.The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200528	60080	10 ug	$500.50		Add to Cart

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