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Home / Proteins & Peptide

PDE7B, GST-tag, His-tag

SKU : BHP18200523

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BPS Bioscience

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Mfr.No.	60071
Description	Human PDE7B GenBank Accession No. NM_018945, (a.a. 109-end) with N-terminal GST-tag and C-terminal His-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=226 pmol/min/ug. Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	109-end
Tag	N-terminal GST-tag, C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE7B kit, Cat. # 60371. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE7B at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_018945
Mw(kda)	66 kDa

Synonyms	PDE7B, phosphodiesterase 7B
Uniprot	Q9NP56

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Gardner,C., et al., Biochem. Biophys. Res. Commun. 272 (1), 186-192 (2000) 2. Sasaki,T., et al., Biochem. Biophys. Res. Commun. 271 (3), 575-583 (2000)Application Reference(s): Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200523	60071	10 ug	$500.50		Add to Cart

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