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Home / Proteins & Peptide

PDE6C, GST-tag

SKU : BHP18200519

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BPS Bioscience

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Mfr.No.	60062
Description	Human PDE6C (GenBank Accession No. NM_006204), full length, with N-terminal GST-tag, MW=125 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 3,000 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM Glutathione, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cGMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE6C at 37°C for 60 min.Quantified by 5'- nucleotidase cleaving the5' -GMP product and releasing thephosphate group which is detected byMalachite Green Reagent. This product is a more active version of former BPS Cat. #60060. Avoid freeze/thaw cycles.
GenBank	NM_006204
Mw(kda)	125 kDa

Synonyms	PDE6C, phosphodiesterase 6C
Uniprot	P51160

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Martinez S.E. et al., J Biol Chem. 2008 Sep 19;283(38):25913-9. 2. Stearns G. et al., J Neurosci. 2007 Dec 12;27(50):13866-74.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200519	60062	5 ug	$500.50		Add to Cart

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