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Home / Proteins & Peptide

PDE6C, GST-Tag (Mouse)

SKU : BHP18200521

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BPS Bioscience

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Mfr.No.	60065
Description	Mouse PDE6C, GenBank Accession No. NM_033614, 2-861 (end), with N-terminal GST tag, MW = 126 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 600 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3´, 5´-cGMP to 5´-GMP per min at 37°C.
Immunogen Region	2-861(end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 10 mM Glutathione, 3 mM DTT and 20% Glycerol
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 uM cGMP, and serial dilutions of PDE6C at 37°C for 20 min. Quantified by 5´ nucleotidase cleaving the 5´-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_033614
Mw(kda)	126 kDa

Synonyms	PDE6, PDE6C, PDE6C Mouse

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.

Reference	1. Martinez S.E. et al., J Biol Chem. 2008 Sep 19;283(38):25913-9.2. Stearns G. et al., J Neurosci. 2007 Dec 12; 27(50):13866-74.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200521	60065	5 ug	$506		Add to Cart

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