JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

PDE5A1, GST-tag

SKU : BHP18200514

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	60050
Description	Human PDE5A1 (GenBank Accession No. NM_001083), full length, with N-terminal GST-tag, MW=126 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 3100 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM DTT, 2.4 mM KCl, 18 mMglutathione, 3 mM DTT and 10% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE5A kit, Cat. # 60350. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cGMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE5A1 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -GMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_001083
Mw(kda)	126 kDa

Synonyms	PDE5A, phosphodiesterase 5A
Uniprot	O76074

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Filippi,S. et al., Endocrinology 148 (3), 1019-1029 (2007). 2. Bruder,S. et al., J. Biol. Chem. 281 (29), 19969-19976 (2006).Application Reference(s): 1. Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015) 2. Active Site Coupling in PDE:PKA Complexes Promotes Resetting of Mammalian cAMP Signaling (2014) 3. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014) 4. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013) 5. Analysis of substrate specificity and kinetics of cyclic nucleotide phosphodiesterases with N'-methylanthraniloyl-substituted purine and pyrimidine 3',5'-cyclic nucleotides by fluorescence spectrometry (2013) 6. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18200514	60050	10 ug	$528		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.