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Home / Proteins & Peptide

PDE4D3, GST-tag

SKU : BHP18200510

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BPS Bioscience

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Mfr.No.	60046
Description	Human PDE4D3 (GenBank Accession No. NM_006203), full length, with N-terminal GST-tag, MW=102.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 12,900 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 3 mM DTTand 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4D3 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_006203
Mw(kda)	102.4 kDa

Synonyms	PDE4D3, phosphodiesterase 4D3
Uniprot	Q08499

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Fidani,L., et al., Eur. J. Neurol. 14 (7), 745-749 (2007). 2. Baillie,G.S., et al., Biochem. J. 404 (1), 71-80 (2007).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200510	60046	5 ug	$544.50		Add to Cart

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