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Home / Proteins & Peptide

PDE4C1, GST-tag

SKU : BHP18200509

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BPS Bioscience

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Mfr.No.	60044
Description	Human PDE4C1 (GenBank Accession No. NM_000923), full length, with N-terminal GST-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=3,000 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 1 mM MnCl₂, 200 uM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4C1 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_000923
Mw(kda)	106 kDa

Synonyms	PDE4C, phosphodiesterase 4C
Uniprot	Q08493

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	Wang H. et al., Biochem J. 2007; 408(2):193-201. 2. Sullivan M., Olsen A.S. and Houslay M.D. Cell. Signal. 1999; 11 (10):735-742.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200509	60044	5 ug	$517		Add to Cart

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