Human PDE4B3 (GenBank Accession No. NM_001037340), 2-721 (end), with N-terminal GST tag, MW=108 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
host species: insect
Host species: insect
Host cell line: Sf9 cells
Specific activity: 10,500 pmole/min/ug
Immunogen Region
2-721 (end)
Tag
N-terminal GST tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 uM cAMP, and serial dilutions of PDE4B3 at 37°C for 20 min. Quantified by 5' nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Avoid freeze/thaw cycles.
GenBank
NM_001037340
Mw(kda)
108 kDa
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Ahmed, T., et al., Neuroscience. 117(3), 627-638 (2003).2. Millar, J, et al., Science. 310(5751), 1187-1191 (2005).