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Home / Proteins & Peptide

PDE4A4B, GST-tag

SKU : BHP18200505

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BPS Bioscience

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Mfr.No.	60039
Description	Human PDE4A4B, also known as PDE4A isoform 1 (GenBank Accession No. NM_001111307), a.a. (2-end) with N-terminal GST-tag, MW= 124 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 11,610 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	2-886(end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 4 mM glutathione,3 mM DTT and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE4A Assay Kit, Cat. # 60340. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4A4B at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_001111307
Mw(kda)	124 kDa

Synonyms	PDE4A isoform 1, PDE4A4B
Uniprot	P27815

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Sullivan,M., et al., Journal Biochem. J. 333:693-703 (1998). 2. Huston E, et al., J Cell Sci. 2006 119:3799-810. (2006).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200505	60039	10 ug	$517		Add to Cart

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