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Home / Proteins & Peptide

PDE4A1A, GST-tag

SKU : BHP18200506

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BPS Bioscience

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Mfr.No.	60040
Description	Human PDE4A1A (GenBank Accession No. U97584), full length with N-terminal GST-tag, MW= 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 9,400 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE4A Assay Kit, Cat. # 60340. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4A1A at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	U97584
Mw(kda)	100 kDa

Synonyms	PDE4A1A, phosphodiesterase 4A1A
Uniprot	P27815

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=80%
Storage	At least 6 months at -80°C.

Reference	1. Sullivan,M., et al., Journal Biochem. J. 333:693-703 (1998). 2. Huston, E., et al., J Cell Sci. 119:3799-810. (2006). Application Reference(s): 1. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014) 2. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200506	60040	10 ug	$533.50		Add to Cart

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