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Home / Proteins & Peptide

PDE4A10, GST-tag

SKU : BHP18200504

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Mfr.No.	60038
Description	Human PDE4A10, also known as PDE4A protein isoform 6, transcript variant 3 (GenBank Accession No. NM_001111309), a.a. 2-825(end) with N-terminal GST-tag, MW= 117 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 4,400 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	2-825(end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE4A Assay Kit, Cat. # 60340. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4A10 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_001111309
Mw(kda)	117 kDa

Synonyms	PDE4A protein isoform 6, transcript variant 3, PDE4A10
Uniprot	P27815

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. McCahill, A., et al. Cell Signal. 2008. Nov; 20(11):2071-83. 2. McPhee, I., et al., Cell Signal. 2001. Dec; 13(12):911-8.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200504	60038	10 ug	$517		Add to Cart

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