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Home / Proteins & Peptide

PDE3B, GST-tag

SKU : BHP18200501

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BPS Bioscience

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Mfr.No.	60031
Description	Human PDE3B (GenBank Accession No. NM_000922), a.a. 592-end, with N-terminal GST-tag, MW = 86 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 1270 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	592-end
Tag	N-terminal GST-tag
Formulation	40 mM Tris, pH 8.0, 110mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04%Tween-20, 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE3B Assay Kit, Cat. # 60331. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE3B at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_000922
Mw(kda)	86 kDa

Synonyms	PDE3B, phosphodiesterase 3B
Uniprot	Q14432

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Raymond, D.R., et al., Cell. Signal. 19 (12), 2507-2518 (2007). 2. Palmer, D., et al., J. Biol. Chem. 282 (13), 9411-9419 (2007).Application References: 1.A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013) 2.Cyclic phosphatidic acid stimulates cAMP production and inhibits growth in human colon cancer cells (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200501	60031	10 ug	$500.50		Add to Cart

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