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Home / Proteins & Peptide

PDE3A (Mouse), GST-Tag

SKU : BHP18200503

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BPS Bioscience

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Mfr.No.	60036
Description	Mouse PDE3A (GenBank Accession No. NM_018779), amino acids 484-1141 end), with N-terminal GST tag, MW = 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=280 pmol/min/ug
Immunogen Region	484-1141
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 18 mM Glutathione
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 uM cAMP, and serial dilutions of Mouse PDE3A at 37°C for 20 min. Quantified by 5' nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_018779
Mw(kda)	100 kDa

Synonyms	PDE3A, phosphodiesterase 3A, PDE3
Uniprot	B2RR84

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Reference	1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200503	60036	5 ug	$500.50		Add to Cart

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