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Home / Proteins & Peptide

PDE3A (Dog), FLAG-Tag

SKU : BHP18200923

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BPS Bioscience

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Mfr.No.	100264
Description	Dog PDE3A, also known as Phosphodiesterase 3A, CGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A, and CGI-PDE A, GenBank Accession No. XP_005637137, a.a. 343-998 (end), with C-terminal FLAG-tag, expressed in a HEK293 expression system, MW = 75 KDa.
Immunogen Region	343-998 (end)
Tag	FLAG-Tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% Glycerol.
Species	Dog
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 uM cAMP, 0.02% TritonX, and serial dilutions of PDE3A at 37°C for 20 min. Quantified by 5? nucleotidase cleaving the 5?-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Absorbance was measured at 620 nm. Avoid multiple freeze/thaw cycles.
GenBank	XP_005637137
Mw(kda)	75 kDa

Synonyms	Phosphodiesterase 3A, cGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A, and CGI-PDE A
Uniprot	F1PL79

Shipping Temperature	-80°C (dry ice)
Format	Aqueous solution
Storage	At least 6 months at -80°C.

Reference	1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006) 2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200923	100264	5 ug	$517		Add to Cart

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