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Home / Proteins & Peptide

PDE2A V2 (Mouse), GST-tag

SKU : BHP18200496

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BPS Bioscience

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Mfr.No.	60018
Description	Murine PDE2A, variant 2 (GenBank Accession No. NM_001008548), a.a 2-935, with N-terminal GST tag, MW=132 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=20,000 pmole/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3?, 5?-cAMP to 5?-AMP per min at 37°C.
Immunogen Region	2-935 (end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 1.6 mM glutathione, 20% glycerol
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 uM cAMP, and serial dilutions of PDE2A at 37°C for 20 min. Quantified by 5? nucleotidase cleaving the 5?-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_001008548
Mw(kda)	132 kDa

Uniprot	O00408

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Reference	1. Sadhu, K., et al., JHC 47(7), 889-894 (1999).2. Iffland, A., et al., Biochemistry 44 (23), 8312-8325 (2005).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200496	60018	5 ug	$500.50		Add to Cart

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