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Home / Proteins & Peptide

PDE2A, GST-tag

SKU : BHP18200498

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BPS Bioscience

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Mfr.No.	60020
Description	Human PDE2A1 (GenBank Accession No. NM_002599), full length, with N-terminal GST-tag, MW=132 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 3442 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE2A Assay Kit, Cat. # 60320. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE2A at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_002599
Mw(kda)	132 kDa

Synonyms	PDE2A, phosphodiesterase 2A
Uniprot	O00408

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Seybold, J., et al., Blood 105 (9), 3569- 3576 (2005). 2. Martinez, S.E., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13260-13265 (2002).Application Reference(s): Active Site Coupling in PDE:PKA Complexes Promotes Resetting of Mammalian cAMP Signaling (2014)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200498	60020	5 ug	$500.50		Add to Cart

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