Human PDE2A1 (GenBank Accession No. NM_002599), a.a. 2-941, with N-terminal FLAG-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: >= 7577 pmol/min/ug
Immunogen Region
2-941
Tag
N-terminal FLAG-tag
Formulation
45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 90 ug/ml FLAGpeptide, 10% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
10 mM Tris-HCl, pH7.4, 10 mM MgCl2, 0.05% Tween-20,200 uM cAMP, and PDE2A1 at 37°C for20 min. Quantified by 5-nucleotidasecleaving the 5-AMP product andreleasing the phosphate group which isdetected by Malachite Green Reagent.
Avoid freeze/thaw cycles.
GenBank
NM_002599
Mw(kda)
106 kDa
Synonyms
PDE2A, phosphodiesterase 2A
Uniprot
O00408
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Seybold, J., et al., Blood 105 (9), 3569-3576 (2005). 2. Martinez, S.E., et al., Proc. Natl. Acad.Sci. U.S.A. 99 (20), 13260-13265(2002).