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Home / Proteins & Peptide

PDE1C, GST-tag

SKU : BHP18200494

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BPS Bioscience

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Mfr.No.	60013
Description	Human PDE1C (GenBank Accession No. NM_005020), a.a. 2-634(end), with N-terminal GST-tag, MW=98 kDa, expressed in a Baculovirus-infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 15,000 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	2-634(end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween-20, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE1C Assay Kit, Cat. # 60312. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE1C at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_005020
Mw(kda)	98 kDa

Synonyms	PDE1C, phosphodiesterase 1C
Uniprot	Q14123

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Vandeput, F., et al. (2007). J. Biol. Chem. Nov 9;282(45):32749-57. 2. Cygnar, K.D., Zhao, H. (2009). Nat. Neurosci. Apr;12(4):454-62. 3. Ortiz-Capisano, M.C., et al. (2009). Am. J. Physiol. Regul. Integr. Comp. Physiol. Nov;297(5):R1469-76.Application Reference(s): A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200494	60013	10 ug	$500.50		Add to Cart

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