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Home / Proteins & Peptide

PDE1B, GST-Tag (Mouse)

SKU : BHP18200016

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BPS Bioscience

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Mfr.No.	11665
Description	Mouse PDE1B (GenBank Accession No. NM_008800), a.a. 42-535 (end), with N-terminal GST-tag, MW= 83 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: insect Host species: insect Host cell line: Sf9 cells Specific activity: 5000 pmol/min/ug. Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3?, 5?-cAMP to 5?-AMP per min at 37°C.
Immunogen Region	42-535 (end)
Tag	N-terminal GST tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol.
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 uM cAMP, and serial dilutions of PDE1B Mouse at 37°C for 20 min. Quantified by 5? nucleotidase cleaving the 5?-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_008800
Mw(kda)	83kDA

Synonyms	DE1B1, PDES1B, HEL-S-79p, phosphodiesterase 1B
Uniprot	Q01065

Shipping Temperature	-80°C (dry ice)
Format	Aqueous solution
Storage	At least 6 months at -80°C.

Reference	1. Spence, S., Biomedical Journal, 321(1),:157-163 (1997).2. Xin, J. et al., Proc. Natl. Acad. Sci. U.S.A. 93: 11236-11241 (1996).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200016	11665	10 ug	$500.50		Add to Cart

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