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Home / Proteins & Peptide

PDE11A, GST-Tag (Mouse)

SKU : BHP18200520

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BPS Bioscience

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Mfr.No.	60064
Description	Mouse PDE11A (GenBank Accession No. NM_001081033), a.a. 2-933(end) with N-terminal GST tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=1,560 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per min at 37°C.
Immunogen Region	2-933(end)
Tag	N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM glutathione, 3 mM DTT, 10% glycerol
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 uM cAMP, and serial dilutions of PDE11A at 37°C for 20 min. Quantified by 5' nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_001081033
Mw(kda)	131 kDa

Synonyms	PDE11, phosphodiesterase 11A

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.

Reference	1. Fawcett, L. et al., PNAS. 97(7), 3207-3707 (2000).2. Horvath, A. et al., Nature Genetics. 38, 794-800 (2006).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200520	60064	5 ug	$506		Add to Cart

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