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Home / Proteins & Peptide

PDE10A2, GST-tag

SKU : BHP18200535

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BPS Bioscience

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Mfr.No.	60100
Description	Human PDE10A2 (GenBank Accession No. NM_006661), full length with N-terminal GST-tag, MW= 116 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 Specific activity: >= 1600 pmol/min/ug Unit definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3' , 5' -cAMP to5' -AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween,3mM DTT, and 20% Glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl, pH7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, andserial dilutions of PDE3A at 37°C for 20min. Quantified by 5 nucleotidasecleaving the 5-AMP product andreleasing the phosphate group which isdetected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_006661
Mw(kda)	116 kDa

Synonyms	PDE10A2, phosphodiesterase 10A2
Uniprot	Q9Y233

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006). 2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200535	60100	10 ug	$500.50		Add to Cart

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