Human PDE10A1 (GenBank Accession No. NM_001130690), a.a. 2-789(end) with N-terminal GST-tag, MW=116 kDa, expressed in Sf9 cells via a Baculovirus expression system.
Host cell line: Sf9
Specific activity: >= 3200 pmol/min/ug
Unit definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3' , 5' -cAMP to5' -AMP per minute at 37°C.
Immunogen Region
2-789(end)
Tag
N-terminal GST-tag
Formulation
45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 20 mMglutathione, 3 mM DTT and 10% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, and 0.25-4 ng/ul PDE10A1 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Avoid freeze/thaw cycles.
GenBank
NM_001130690
Mw(kda)
116 kDa
Synonyms
PDE10A1, phosphodiesterase 10A1
Uniprot
Q9Y233
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006). 2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).Product Specific Reference(s): 1. Demirbas, D., et al., PLoS One. 8(8), (2013). 2. Wang, Z., et al., J Sex Med. 10(11), 2790-7 (2013).