Human secreted Programmed Death Ligand 2 (PD-L2)-Fc fusion protein, also known as CD273, PDCDL1G2, and B7-DC, GenBank Accession No. NM_025239, a.a. 20-219, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avitag expressed in a HEK293 cell expression system and enzymatically biotinlabeled using Avi-tag technology. MW = 51 kDa.
Immunogen Region
20-219
Tag
C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Species
Human
Application
Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Notes
PD-1-PD-L2 binding activity, measuredusing the using the PD-1:PDL2(Biotinylated) Inhibitor Screening AssayKit, Biohippo, Catalog #72004.
This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.