Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7-H1) with a K124A mutation, GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus with the Fc portion of human IgG1, with C-terminal Avi-tag expressed in a HEK293 cell expression system. MW = 54 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
19-239
Tag
C-terminal Avi-tag
Formulation
8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol
Species
Human
Application
Useful for the study of protein-protein interaction, epitope mapping, and in vitro and in vivo protein function.
Notes
HiP indicates a high purity protein with>90 percent purity by gel filtration. This protein runs at a higher MW by SDS-PAGE due to glycosylation. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_014143
Mw(kda)
54 kDa+glycans
Synonyms
CD274, B7 homolog 1, B7-H1
Uniprot
Q9NZQ7
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Freeman, G.J., et al., J Exp Med. 2000 Oct 2;192(7):1027-34.2. Dong, H., et al., Nat Med. 1999 Dec;5(12):1365-9.