Mouse secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7-H1), GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus with the Fc portion of mouse IgG2a, expressed in a HEK293 cell expression system. MW = 53 kDa.
Immunogen Region
19-239
Tag
Fc Fusion
Formulation
8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol
Species
Mouse
Application
Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Notes
Add 50 uL of PD-1-Biotin in assay buffer (PBS with 0.1% BSA) to the wells precoated with PD-L1 on a white maxisorp plate. Incubate the reaction mixture at room temperature for 2 hours. Wash and block wells. Then add streptavidin-HRP and incubate for 1 hour. Finally, add HRP chemiluminescent substrates and read luminescence.
Avoid freeze/thaw cycles.
GenBank
NM_014143
Mw(kda)
53 kDa
Synonyms
CD274, B7 homolog 1, B7-H1, B7H1
Uniprot
Q9NZQ7
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.