Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274, B7 homolog 1 (B7- H1), and PD-L1 Biotin-Labeled. GenBank Accession No. NM_014143, amino acids 19-239, fused at the Cterminus with the Fc portion of human IgG1, with C-terminal Avi-tag expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 54 kDa.
Immunogen Region
19-239
Tag
C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Human
Application
Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Notes
PD-1-PD-L1 binding activity, measured using the using the PD-1:PDL1 (Biotinylated) Inhibitor Screening Assay Kit, Biohippo, Catalog #72003.
HiP indicates a high purity protein with>=90% purity as measured by gel filtration. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.