Human secreted Programmed Cell Death 1(PD-1)-Fc fusion protein, also known asCD279, PDCD1, and SLEB2, GenBankAccession No. NM_014143, a.a. 25-167with a K78A mutation, fused at the Cterminuswith the Fc portion of human IgG1,and C-terminal AviTag, expressed in aHEK293 cell expression system andenzymatically biotin-labeled using AviTagtechnology. MW = 44.5 kDa. This proteinruns at a higher MW by SDS-PAGE due toglycosylation.
Formulation
8 mM phosphate, pH 7.4,110 mM NaCl, 2.2 mM KCl, and 20%glycerol
Species
Human
Application
Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Notes
The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_014143
Mw(kda)
44.5 kDa+glycans
Synonyms
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L
Uniprot
Q15116
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Freeman, G.J., et al., J. Exp. Med. 2000 Oct 2;192(7):1027-34.2. Dong, H., et al., Nat Med. 1999. Dec;5(12):1365-9.