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Home / Proteins & Peptide

PD-1 (CD279), Fc fusion, Biotin-labeled (Human) HiP

SKU : BHP18201544

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Mfr.No.	71109-3
Description	Human secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279, HPD-L, PD-1 Biotin-labeled GenBank Accession No. NM_005018, a.a 25-167, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avitag expressed in a HEK293 cell expression system and enzymatically biotinlabeled using Avi-tag technology. MW = 44.5 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Immunogen Region	25-167
Tag	C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species	Human
Application	Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Notes	BPS Cat. 72005protocol was followed. Briefly, 50 ul of 2ug/ml PD-L1 was coated onto plates. 20 ulof 0.5 ug/ml PD-1, Biotin-labeled wasallowed to bind at room temp. for 2 hours.Strep-HRP was added and allowed toincubate at room temp for 1 hr, followed byHPR chemiluminescent substrate additionand plate reading. HiP indicates a high purity protein with>=90% purity as measured by gel filtration. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank	NM_005018
Mw(kda)	44.5 kDa

Synonyms	Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L
Uniprot	Q15116

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6. 2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201544	71109-3	500 ug	$4499		Add to Cart

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