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Home / Proteins & Peptide

PCSK9, His-tag

SKU : BHP18201589

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Mfr.No.	71204-1
Description	Human proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as FH3, HCHOLA3, and PC9, GenBank Accession No. NM_174936, a.a. 31-692(end), with C-terminal His-tag, MW=71.8 kDa (calculated), expressed in an HEK293 cell expression system.
Background	PCSK9 is a crucial player in the regulation of plasma cholesterol homeostasis. It binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Immunogen Region	31-692(end)
Tag	C-terminal His-tag
Formulation	8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species	Human
Application	Useful for studying protein binding and screening small molecules for drug discovery.
Notes	Coated plate with 100 ng/well of PCSK9-his (#71204) in 50 ul (in PBS) (overnight at 4 deg). Initiated binding reaction with addition of various concentrations of LDLR-Biotin in 50 mM MES pH6, 50 mM NaCl, 0. 2 mM CaCl2, 0.5% Tween-20, and 0.1% BSA (AKA PCSK9-LDLR TR-FRET assay buffer). Incubated for 2h at RT. Detected binding with Strep-HRP generated luminescence. PCSK9 is autocleaved to the ~14 kDa prodomain (a.a. 31-152) and the ~60 kDa mature form (a.a. 153-692(end)), which run at higher apparent MW by SDS-PAGE. Avoid freeze/thaw cycles.
GenBank	NM_174936
Mw(kda)	71.8 kDa

Synonyms	FH3, HCHOLA3, PC9, NARC1, Neural Apoptosis Regulated Convertase 1
Uniprot	Q8NBP7

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Abifadel, M., et al., Nat Genet. 2003 Jun,34(2):154-6. 2. Abifadel, M., et al., Hum Mutat. 2009 Apr,30(4):520-9.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201589	71204-1	20 ug	$335.50		Add to Cart

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