Human PARP3 (GenBank Accession No. NM_001003931), full length with N-terminal GST-tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Unit definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted usingBPS's PARP3 Assay Kit (Catalog #80553) at room temperature for 1 hour.