Human poly (ADP-ribose) polymerase family, member 15 (PARP15) (GenBank Accession No. NM_152615, var.2), a.a. 221-444(end) with N-terminal GST-tag, MW = 52.5 kDa, expressed in an E. coli expression system.
Unit definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Immunogen Region
221-444(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted usingBPS's PARP15 Assay Kit (Catalog #80567) at room temperature for 30 minutes.