Human PARP1 (GenBankAccession No. NM_001618), full lengthwith N-terminal FLAG-Avi-tag, MW = 117kDa, expressed in a Baculovirus infectedSf9 cell expression system.
Immunogen Region
Full Length
Tag
N-terminal FLAG-Avi-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 0.2mM TCEP, 80 ug/ml FLAG peptide
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction isconducted using BPS PARP Assay Kit(Catalog Number 80551) at roomtemperature for 1 hour.
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).