Human PADI2, also known as Peptidyl arginine deiminase, type II, (GenBank Accession No. NM_007365), 1-665(end), with C-terminal FLAG-tag, MW= 77 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: >=19 pmole/min/ug.
Immunogen Region
1-665(end)
Tag
C-terminal FLAG-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/ul FLAG peptide, 3 mMDTT, and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50mM NaCl, 10 mM CaCl2, and 2 mM DTT)containing PADI and 10 mM BAEE as asubstrate for 30 min at 37°C.
Avoid freeze/thaw cycles.
GenBank
NM_007365
Mw(kda)
77 kDa
Synonyms
PADI-2, Peptidyl arginine deiminase, type II, Pad
Uniprot
Q9Y2J8
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Dong S, et al. (2005.) J. Invest. Dermatol. 124(5):1026-33. 2. Wantanabe Y, et al. (2009). J Hum Genet. 54 (7): 430-2.