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Home / Proteins & Peptide

p70S6K (S6K1), GST-tag

SKU : BHP18200149

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BPS Bioscience

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Mfr.No.	40062
Description	Human p70S6Kb1, also known as ribosomal protein S6 kinase, 70 kDa, polypeptide 1 (RPS6KB1), GenBank Accession No. NM_003161, amino acid 1-421 with N-terminal GST tag, MW=74 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 2 pmol/min/ug
Background	p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6k activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389 a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).
Immunogen Region	1-421
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35 and 2 uM substrate (SER/THR 7, Invitrogen) at room temperature for 1 hour. Avoid freeze/thaw cycles.
GenBank	NM_003161
Mw(kda)	74 kDa

Synonyms	RPS6KB1, p70S6K, Ribosomal S6 kinase, S6K, STK14A
Uniprot	P23443

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Di Paolo S. et al., Am. J. Kidney Dis. 49 (3), 462-470 (2007). 2. Fang C.X. et al., J. Exp. Neurol. 203 (1), 196-204 (2007).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200149	40062	20 ug	$412.50		Add to Cart

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