Recombinant human p53(tumor protein p53), full length, encompassing amino acids 2-393(end). In this construct, the N-terminal end of the protein is fused to GST via a short peptide linker placed between GST consisting of an optimal TEV protease target sequence that allows cleavage of GST (TEV: tobacco etch virus cysteine proteaseBiohippo #50308). The construct also contains mutation of interest (Y220C). The recombinant protein was affinity purified.
host species: Sf9
Host species: Sf9
Immunogen Region
2-393(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol
Species
Human
Notes
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles. Avoid freeze/thaw cycles