Human E1A binding protein p300, also known as EP300, KAT3B, or RSTS2, GenBank Accession No. NM_001429, a.a. 965-1810, with N-terminal His-GST-tag, MW=125 kDa, expressed in Sf9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: >=357 pmole/min/ug
Immunogen Region
965-1810
Tag
N-terminal His-GST-tags
Formulation
40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 200 mMimidazole, and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assayed with the BiohippoFluorogenic p300 Assay Kit, Cat. #50092, per manufacturer's instructions.
Avoid freeze/thaw cycles.
GenBank
NM_001429
Mw(kda)
125 kDa
Synonyms
EP300, KAT3B, RSTS2, E1A binding protein
Uniprot
Q09472
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Gui, Y., et al., Nat. Genet. 2011 Aug 7; 43(9) : 875 - 878. 2. Morin, R.D., et al., Nature. 2011 Jul 27; 476(7360) : 298 - 303. 3. Zhao, Z., et al., Brain Res. Bull. 2011 Oct 10; 86(3-4) : 189 - 194.Product Specific Reference(s): 1. Furdas, S.D. et al., Med. Chem. Commun. 2014; 5: 1856-1862. 2. Liao, D., et al., Cancer. Epigenetics. 2005; 1238: 539-548.