JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

p110alpha(K111N)/p85alpha, FLAG-tag

SKU : BHP18200285

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	40639
Description	Complex of N-terminal FLAG-tagged recombinant full-length human p110alpha (GenBank Accession No. NM_006218), with K111N mutation, and recombinant full length, human p85alpha (no tag, GenBank Accession No. NM_181523). Co-expressed in Sf9 cells in aBaculovirus expression system. p110alpha(K111N) MW=124 kDa, p85alpha MW=84 kDa. Host cell line: Sf9 cells Specific activity: 1326 pmol/min/ug.
Immunogen Region	full length
Tag	FLAG-tag(p110alpha), no tag on p85alpha
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04%Tween-20, 20% glycerol, 0.2 mM TCEP,80 ug/ml flag peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reactions (25 ul) were doneat 30°C for 15 min using BPS PI3K substrate,and the samples were incubated with the ADPGloreagent (25 ul) for 45 min followed byincubation with the Kinase Detection reagent(50 ul) for 55 min. Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	NM_006218 (p110alpha), NM_181523 (p85alpha)
Mw(kda)	208 kDa (complex)

Synonyms	PIK3CA, p110alpha, Phosphoinositide-3-Kinase, Catalytic, Alpha, PIK3R1, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), GRB1, p85alpha
Uniprot	p110alpha: P42336; p85alpha: P27986

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Rudd, M.L., et al., Clin. Canc. Res. 2011; 17(6): 1331-1340. 2. Brady, S.W., et al., Molec. Canc. Ther. 2014; 13(1): 60-70.

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18200285	40639	20 ug	$456.50		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.