With enhanced performance for high molecular weight proteins, Opti-Protein Ultra Marker consists of 10 pre-stained proteins that resolve into sharp, tight bands covering a wide range of molecular weights from 6.5 to 270 kDa. This protein ladder allows you to monitor protein separation during electrophoresis, estimate molecular weight of the protein of interest, and evaluate weste blot transfer efficiency. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on gel.
Suggested Load
Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Features
- Broad range: 6.5 to 270 kDa - Ready-to-use: Load directly into gel - Easy identification: 3 colored reference bands (orange at 30 kDa and 270 kDa and green at 52 kDa) - Clean/Sharp bands: 8 sharp markers visible at 270, 175, 130, 95, 66, 52, 37, and 30 kDa and 2 additional bands at 16 and 6.5 kDa (Tris-Glycine 4-20% Gel)
Quantity
500 µl/100 loads
Range
6.5 kDa-270 kDa
Number Of Bands
Resolves into 10 sharp, tight bands.
Application
• Monitoring protein migration during SDS-PAGE • Monitoring efficiency of protein transfer onto PVDF or nitrocellulose for Weste Blotting • Sizing of proteins on SDS-PAGE and Weste Blots