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Home / Proteins & Peptide

NSP8_NSP7/NSP12 (SARS-CoV-2) Complex Recombinant

SKU : BHP18201082

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BPS Bioscience

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Mfr.No.	101466
Description	Recombinant complex of three SARS-CoV-2 proteins NSP7 (nonstructural protein 7), NSP8 (nonstructural protein 8) and NSP12 (nonstructural protein 12). The NSP8_NSP7 construct contains an N-terminal His-tag (14xHis) followed by a TEV protease target sequence that allows cleavage of His-tag (TEV: tobacco etch virus cysteine protease Biohippo #50308). The recombinant NSP12 construct is full length encompassing amino acids 1-932 (end). These recombinant proteins are co-expressed in an E. coli expression system and affinity purified. host species: E. coli Host species: E. coli
Immunogen Region	NSP8_NSP7: 1-242(end); NSP12: 1-932(end)
Tag	NSP8_NSP7: N-terminal His-tag
Formulation	20 mM HEPES, pH 8.0, 300 mM NaCl, 10% glycerol, and 3 mM DTT
Species	SARS-CoV-2
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	RNA-dependent RNA polymerase was incubated with FRET Dig-RNA Duplex or Eu-RNA Duplex in the presence of RNaseOUT and biotinylated ATP for 60 min at 37°C in a 10-ul reaction. Streptavidin-acceptor dye and Eu-labeled a-Dig antibody (for Dig-labeled substrate) was then added followed by TR-FRET detection (317 => 620 nm, and 317 => 665 nm). Increase in TR-FRET is proportional to the amount of ATP incorporated in the RNA. Avoid freeze/thaw cycles.
GenBank	NSP7: YP_009742614; NSP8: YP 009725304.1; NSP12: YP_009725307
Mw(kda)	NSP12: 108 kDa; NSP8: 23 kDa; NSP7: 10 kDa

Synonyms	RdRP, RDR, RNA replicase, SARS-coronavirus RNA polymerase, nsp12, nonstructural protein 8, SARS-CoV-2 NSP8, ncov NSP8, NSP 8, NSP-8, nonstructural protein 7, SARS-CoV-2 NSP7, ncov NSP7, NSP 7, NSP-7

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201082	101466	20 ug	$544.50		Add to Cart

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